C57BL/6 • 310680
| Product name | B-luc B16-F10 |
|---|---|
| Catalog number | 310680 |
| Strain name | C57BL/6 |
| Strain background | C57BL/6 |
| Aliases | NA |
| Tissue | Skin |
| Disease | Melanoma |
| Species | Mouse |
This B-luc B16-F10 cell line expresses firefly luciferase as a marker of B16-F10 cells. Luminescence can be observed B-luc B16-F10 cells.
Gene targeting strategy for B-luc B16-F10. The luciferase cDNA sequence with CAG promoter was inserted in the ROSA26 exon1 of wild-type B16-F10.
Luminescence signal intensity of B-luc B16-F10 cells. Luminescence intensity was measured using the Bright-GloTM luciferase Assay. B-luc B16-F10 cells have a strong luminescence signal. The C5 clone of B-luc B16-F10 cells can be used in vivo experiments.
Subcutaneous homograft tumor growth of B-luc B16-F10 cells. B-luc B16-F10 cells and wild-type B16-F10 cells were subcutaneously implanted into C57BL/6 mice (female, 7-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume. (B) Body weight. Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-luc B16-F10 cells were able to establish tumors in vivo and can be used for efficacy studies. Values are expressed as mean ± SEM.
