B-hGDF8 mice

    C57BL/6N-Gdf8tm1(GDF8)Bcgen/Bcgen • 112413

    B-hGDF8 mice

    Product nameB-hGDF8 mice
    Catalog number112413
    Strain nameC57BL/6N-Gdf8tm1(GDF8)Bcgen/Bcgen
    Strain backgroundC57BL/6N
    NCBI gene ID (Human)
    AliasesGDF8; MSLHP

    在此页面上

    • Targeting strategy
    • Phenotypic analysis
    • Efficacy

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        Targeting strategy

        Gene targeting strategy for B-hGDF8 mice. The exons 1-3 of mouse Gdf8 gene that encodes the full-length protein were replaced by human GDF8 exons 1-3 in B-hGDF8 mice.

        Protein expression analysis

        Strain specific GDF8 expression analysis in homozygous B-hGDF8 mice by ELISA. Plasma was collected from wild-type C57BL/6JNifdc mice (+/+) and homozygous B-hGDF8 mice (H/H) (male, 6-week-old), and analyzed by ELISA. Human and mouse GDF8 proteins were both detectable in wild-type C57BL/6JNifdc and B-hGDF8 mice, but not in B-Gdf8 KO mice. The ELISA kit cross-recognizes human and mouse GDF8.

        mRNA expression analysis
        Strain specific analysis of GDF8 mRNA expression in wild-type C57BL/6 mice and B-hGDF8 mice by RT-PCR. Skeletal muscle RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hGDF8 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human GDF8 primers. Mouse Gdf8 mRNA was detectable only in wild-type C57BL/6 mice. Human GDF8 mRNA was detectable only in homozygous B-hGDF8 mice but not in wild-type mice.
        In Vivo Efficacy Study in HFD Induced B-hGDF8 mice

        In Vivo Efficacy Study in HFD-Induced B-hGDF8 mice. B-hGDF8 mice were fed with a high-fat diet for 14 weeks to induce obesity. (A) Body weight change after treatment. (B) Lean tissue changes from Day-1 analyzed by the body composition analyzer. (C) Fat tissue changes from Day-1 analyzed by the body composition analyzer. Myostatin monoclonal blocking antibody promoted lean tissue growth, and in combination with semaglutide, it prevented semaglutide-induced lean tissue loss. Values are expressed as mean ± SEM.

        Note: Data allowed to be shared by the clients.

        * When publishing results obtained using this animal model, please acknowledge the source as follows: The animal model [B-hGDF8 mice] (Cat# 112413) was purchased from Biocytogen.
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